LOCUS       40924_29526        5807 bp DNA     circular SYN 18-DEC-2018
DEFINITION  S. pombe expression vector pLYS1U DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     A novel series of vectors for chromosomal integration in fission 
            yeast
  JOURNAL   Biochem. Biophys. Res. Commun. 374 (2), 315-319 (2008)
  PUBMED    18634753
REFERENCE   2  (bases 1 to 5807)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Fission yeast integration vector pLYS1U
  JOURNAL   Published Only in Database (2007)
REFERENCE   3  (bases 1 to 5807)
  AUTHORS   Matsuyama A, Shirai A, Yoshida M.
  TITLE     Direct Submission
  JOURNAL   Submitted (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical 
            Genetics Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL
            :http://cgl.riken.go.jp/
REFERENCE   4  (bases 1 to 5807)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 5807)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem. 
            Biophys. Res. Commun."; date: "2008"; volume: "374"; issue: "2"; 
            pages: "315-319"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Published 
            Only in Database (2007)"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (15-OCT-2007) Contact:Akihisa Matsuyama RIKEN, Chemical Genetics 
            Laboratory; Hirosawa 2-1, Wako, Saitama 351-0198, Japan URL 
            :http://cgl.riken.go.jp/"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     Fission yeast expression vector.
FEATURES             Location/Qualifiers
     source          1..5807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      21..208
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae
                     alcohol dehydrogenase 1 (ADH1) gene"
     CDS             242..956
                     /pseudo
                     /gene="lys1"
                     /label=lys1
                     /note="NotI site is created at the middle of this fragment 
                     (613-620)."
     gene            242..956
                     /pseudo
                     /gene="lys1"
                     /label=lys1
     regulatory      959..1481
                     /gene="ura4"
                     /regulatory_class="promoter"
     CDS             1482..2273
                     /label=S. pombe ura4
                     /note="orotidine 5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
     regulatory      2277..2654
                     /gene="ura4"
                     /regulatory_class="terminator"
     primer_bind     complement(2667..2683)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      2896..3351
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3633..3737
                     /label=AmpR promoter
     CDS             3738..4595
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4769..5357
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    5645..5666
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        5681..5711
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5719..5735
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5743..5759
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
       61 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
      121 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
      181 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatatact
      241 agtactttgt agtacctgcg cggtctcaag atcaaacatt tttggaatcg caaaaagatg
      301 tcattccagc cggacgtggc atgaagaatg tccaactttt ggtgatcaat cgttttgata
      361 ctaacaagat atgcggtatc ggagaagttg gtgaaatata tcttcgtgct ggtggattgg
      421 ctgagggcta ccttggaaat gatgaattga ctagcaaaaa attcttaaag agttggtttg
      481 ctgatccctc caagtttgta gaccgaactc cagaaaatgc gccctggaaa ccttattggt
      541 tcggaatacg cgatcgtatg tatcgttctg gtgacttggg ccgctatctc ccaactggta
      601 atgtagaatg tagcggccgc cgacgatcaa attaaaattc gtggtttccg aattgagtta
      661 ggtgaaatta atacccatct ttctcgtcat cctaatgtcc gtgaaaatat tacccttgtt
      721 cgacgtgata aggatgaaga acccacgcta gttgcataca ttgtacctca aggtttaaat
      781 aaagatgact ttgatagcgc tactgagtcg gaagatattg tcgtcaatgg tttgaagaag
      841 tacaggaagc ttatacatga tatacgggaa tatcttaaga ctaaacttcc tagttatgct
      901 attccttctg taattgttcc ccttcataaa atgcctctca atcctaatgg aaagatctac
      961 aaatcccact ggctatatgt atgcatttgt gttaaaaaag tttgtataga ttatttaatc
     1021 tactcagcat tctttctcta aataggaatt tgttacttaa tggagaaaaa aatgtttcga
     1081 tttacctagt gtatttgttt gtatactcac gtttaatttc aaacatccat tctatcttgt
     1141 gtaatttttg gcatggtgaa aaagataatc agccttataa tctttacaaa agtaagaaat
     1201 tctgtaaata agccttaatg cccttgcttt aaattaaaat ggttcttttt catgataatg
     1261 tttgcacttt gtgaatatat tttagatagt tctgtgaggt ataattaaga tgttttagag
     1321 acttatacaa ttttgtcttt ataaattctt aattgatttt accatcccag tttaactatg
     1381 cttcgtcggc atctctgcac atgtcgtgtt ttcttaccgt attgtcctac caagaacctc
     1441 ttttttgctt ggatcgaaat taaaggttta aaagcaaagt tatggatgct agagtatttc
     1501 aaagctattc agctagagct gaggggatga aaaatcccat tgccaaggaa ttgttggctt
     1561 tgatggaaga aaagcaaagc aacttgtcag tcgcggtcga tttgacgaag aaatccgaaa
     1621 tcttagaatt ggtagataaa attggaccct atgtctgtgt tatcaagaca catattgacg
     1681 ttgtcgagga tttcgaccag gatatggtag aaaaactggt ggccttaggt aaaaagcatc
     1741 gttttcttat ctttgaggat cgcaaattcg cagacattgg aaataccgtc aagctacaat
     1801 atgcatctgg tgtgtacaaa attgcttctt gggctcatat cacaaattgc catacagtgc
     1861 caggcgaggg tattatacaa ggcctcaaag aagttggttt acctttggga cgtggtctct
     1921 tgcttttggc tgaaatgtct tccaaaggct ctttggctac tggttcctac acagagaaaa
     1981 ccttagaatg gtttgagaag cataccgatt tttgctttgg ctttatagct ggtcgtcgat
     2041 ttcctaacct tcaaagcgac tacataacta tgtcccctgg tatcggcttg gatgttaaag
     2101 gagacgggct gggacagcaa tatcgtactc ctgaagaagt gattgtaaac tgcggtagcg
     2161 atatcatcat tgttggtcgt ggagtctatg gagctggtcg taatcctgtt gtcgaagcca
     2221 agagatatag agaagctggt tggaaggcat atcagcaaag actttctcag cattaaaaaa
     2281 agactaatgt aaaatttttt tggttggtta ttgaaaaagt cgatgccttg tttgcgtttg
     2341 ttttcctagg cgttttatgt cagaaggcat ttagaattag tatacaagta ctctttggta
     2401 aaattttatg tagcgactaa aatattaact attatagata aacaccttgg gaataaaaag
     2461 taatttgcta tagtaattta ttaaacatgc tcctacaaca ttaccacaat cttttctctt
     2521 ggattgacat tgaataagaa aagagtgaat ttttttagac ttgtaatgat aactatgtac
     2581 aaagccaatg aaagatgtat gtagatgaat gtaaaatacc atgtagacaa acaagataaa
     2641 aatcacgttt aaacgagctc gaattcactg gccgtcgttt tacaacgtcg tgactgggaa
     2701 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     2761 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     2821 tggcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt
     2881 caaagcaacc atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
     2941 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
     3001 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
     3061 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg
     3121 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
     3181 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcgggct
     3241 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
     3301 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca
     3361 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     3421 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     3481 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     3541 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     3601 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     3661 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     3721 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     3781 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     3841 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     3901 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     3961 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     4021 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     4081 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     4141 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     4201 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     4261 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     4321 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     4381 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     4441 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     4501 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     4561 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     4621 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     4681 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     4741 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     4801 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     4861 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
     4921 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     4981 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     5041 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     5101 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     5161 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     5221 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     5281 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     5341 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     5401 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     5461 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     5521 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     5581 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     5641 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     5701 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     5761 atgattacgc caagcttgca tgcctgcagg tcgactctag aggatcc
//
